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Analyse af retinoidsyre-induceret Neural Differentiation af muse embryonale stamceller i to og tre-dimensionelle embryoide legemer
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1Department of Medicine, Cardeza Vascular Research Center,Sidney Kimmel Medical College, Thomas Jefferson University, 2Department of Molecular Cardiology,Cleveland Clinic Foundation, 3Department of Cancer Biology, Cardeza Vascular Research Center,Sidney Kimmel Medical College, Thomas Jefferson University
Chapitres
- 00:05Titre
- 00:51Embryoid Body Formation
- 01:582D Embryoid Body Culture and Detection of Secreted Proteins
- 03:043D Embryoid Body Culture
- 04:06Embryoid Body Dissociation
- 05:56Analysis of Embryoid Body Formation by Immunofluorescence
- 07:12Results: Characteristics of Embryo Body Cultures and Visualization of Neural Differentiation Markers
- 08:22Conclusion
Vi beskriver en teknik til anvendelse af murine embryonale stamceller til generering af to- eller tredimensionale embryoide legemer. Vi derefter forklare, hvordan at inducere neural differentiering af embryoide kropsceller ved retinsyre, og hvordan man analyserer deres tilstand af differentiering ved progenitorcelle markør immunofluorescens og immunoblotting.
