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对于电生理和钙成像急性神经组织的长时间培养
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Neurosciences
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Journal JoVE Neurosciences
Prolonged Incubation of Acute Neuronal Tissue for Electrophysiology and Calcium-imaging
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对于电生理和钙成像急性神经组织的长时间培养

11,467 Views

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10:30 min

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February 15, 2017

DOI:

10.3791/55396-v

DOI:
10.3791/55396-v

•

10:30 min
February 15, 2017

•

35 Views
, , , , , , , ,
1The MARCS Institute,Western Sydney University, 2School of Medicine,Western Sydney University

Summary

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一旦从主体去除，神经元组织大大受环境条件的影响，从而导致组织的最终降解后6 - 8小时。采用了独特的培养方法，该方法密切监测和调节组织的胞外环境中，组织的生存力可以显著延长> 24小时。

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Cite this Article

Cameron, M. A., Kekesi, O., Morley, J. W., Bellot-Saez, A., Kueh, S., Breen, P., van Schaik, A., Tapson, J., Buskila, Y. Prolonged Incubation of Acute Neuronal Tissue for Electrophysiology and Calcium-imaging. J. Vis. Exp. (120), e55396, doi:10.3791/55396 (2017).

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