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Measuring and Altering Mating Drive in Male Drosophila melanogaster
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Neurosciences
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Journal JoVE Neurosciences
Measuring and Altering Mating Drive in Male Drosophila melanogaster

Measuring and Altering Mating Drive in Male Drosophila melanogaster

DOI:
10.3791/55291-v

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07:02 min

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February 15, 2017

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, , ,
1F.M. Kirby Neurobiology Center,Boston Children’s Hospital, 2Harvard NeuroDiscovery Center,Harvard Medical School, 3Department of Neurobiology,Harvard Medical School

Chapitres

  • 00:05Titre
  • 00:38Preparing Virgin Females for Behavioral Assays
  • 01:27Food Preparation for 2-D Satiety Assay
  • 02:36Performing and Scoring 2-D Satiety Assays
  • 04:10Using Courtship to Verify Satiety
  • 04:58Recovering Mating Drive after 2-D Satiety Assay
  • 05:29Results: Reversible Decline in Male Mating Drive over Time
  • 06:31Conclusion

Summary

Traduction automatique

Traduction automatique

This article describes a behavioral assay that uses male mating drive in Drosophila melanogaster to study motivation. Using this method, researchers can utilize advanced fly neurogenetic techniques to uncover the genetic, molecular, and cellular mechanisms that underlie this motivation.

Tags

Keywords: Drosophila MelanogasterMating DriveMotivationHigh-throughput Assay2-D Satiety AssayVirgin FemalesGenetic ToolsHeat ShockFood PreparationIncubatorHumidity Control

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