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JoVE Journal Developmental Biology
Stimulation of Notch Signaling in Mouse Osteoclast Precursors
 

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Article DOI: 10.3791/55234-v • 08:01 min • February 28th, 2017
February 28th, 2017 •
1,2, 1, 1,3, 1,4
1Department of Orthopaedic Surgery, Perelman School of Medicine, University of Pennsylvania, 2Department of Biological Sciences, University of the Sciences, 3The Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, 4Department of Biology, College of Science, Technology, Engineering, & Mathematics, Eastern Washington University

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résumé
Automatic Translation

Notch signaling is a form of cellular communication that relies upon direct contact between cells. To properly induce Notch signaling in vitro, Notch ligands must be presented to cells in an immobilized state. This protocol describes methods for in vitro stimulation of Notch signaling in mouse osteoclast precursors.

Tags

Notch Signaling Osteoclast Precursors Cell Biology Cellular Process Notch Ligands Osteoclast Differentiation Cell Survival Cell Proliferation Mouse Osteoclasts Mouse Hind Limb Tibia And Femur Incision Bone Marrow
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