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共免疫沈降により核および細胞質画分中のタンパク質間相互作用の可視化と<em>その場で</em>近接ライゲーションアッセイ
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Biochimie
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Journal JoVE Biochimie
Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
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共免疫沈降により核および細胞質画分中のタンパク質間相互作用の可視化と<em>その場で</em>近接ライゲーションアッセイ

12,467 Views

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10:05 min

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January 16, 2017

DOI:

10.3791/55218-v

DOI:
10.3791/55218-v

•

10:05 min
January 16, 2017

•

4 Views
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1University Children’s Hospital Basel (UKBB),University of Basel, 2Department of Clinical Research,University of Basel

Tags

Protein-protein InteractionCo-immunoprecipitationIn Situ Proximity Ligation AssayNuclear FractionCytoplasmic FractionCellular CompartmentsHEK293 CellsCytoplasmic ExtractionNuclear ExtractionProtein G Magnetic BeadsInput Samples

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Zhu, X., Zelmer, A., Wellmann, S. Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay. J. Vis. Exp. (119), e55218, doi:10.3791/55218 (2017).

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