MicroScale Thermophoresis (MST) is a sensitive technology to characterize aptamer-target interactions. This manuscript describes an MST protocol to characterize aptamer-small molecule interactions.
Entzian, C., Schubert, T. Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis. J. Vis. Exp. (119), e55070, doi:10.3791/55070 (2017).