Targeted manipulations to cause directed stress or death in individual cells have been relatively difficult to accomplish. Here, a single-cell-resolution ablation approach to selectively stress and kill individual cells in cell culture and living animals is described based on a standard confocal UV laser.
Morsch, M., Radford, R. A. W., Don, E. K., Lee, A., Hortle, E., Cole, N. J., Chung, R. S. Triggering Cell Stress and Death Using Conventional UV Laser Confocal Microscopy. J. Vis. Exp. (120), e54983, doi:10.3791/54983 (2017).