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Isolamento e la coltura di adulti neurali cellule staminali dal Subcallosal Zona mouse
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Isolation and Culture of Adult Neural Stem Cells from the Mouse Subcallosal Zone
DOI:

09:24 min

December 15, 2016

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Chapitres

  • 00:05Titre
  • 00:49Preparation of Medium and Coating Plate
  • 02:09Isolation and Dissociation of the Adult SCZ
  • 03:38SCZ-derived aNSC Culture
  • 05:18Passaging and Differentiation of SCZ-aNSCs
  • 07:22Results: Maintenance of the SCZ-aNSCs as a Monolayer Culture
  • 08:17Conclusion

Summary

Traduction automatique

Establishing culture systems for the expansion of adult neural stem cells (aNSCs) allows for the examination and application of aNSCs for therapy. The subcallosal zone (SCZ) has recently been recognized as a novel neuroblast-forming region in adult mice. Here, methods for the isolation, expansion, and differentiation of SCZ-aNSCs are described.

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