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Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining
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Chapitres
- 00:05Titre
- 01:16Protein Kinase Inhibitor (PKI) Treatment and Fluorochrome-labeled Peptide (p)MHC-I Tetramer Staining
- 02:30Cell Surface Staining, Fixation, Permeabilization and Intracellular Staining
- 04:05Results: Representative pMHC-I Tetramer Staining
- 05:17Conclusion
Here, we present an optimized protocol for enumerating and characterizing rhesus macaque CD8+ T cells against the AIDS virus. This article is useful not only to the field of HIV immunology, but also to other areas of biomedical research where CD8+ T cell responses are known to affect disease outcome.
