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Immunologie et infection
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Detection of Trypanosoma brucei Variant Surface Glycoprotein Switching by Magnetic Activated Cell Sorting and Flow Cytometry
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Immunologie et infection
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Journal JoVE Immunologie et infection
Detection of Trypanosoma brucei Variant Surface Glycoprotein Switching by Magnetic Activated Cell Sorting and Flow Cytometry

Detection of Trypanosoma brucei Variant Surface Glycoprotein Switching by Magnetic Activated Cell Sorting and Flow Cytometry

8,596 Views

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09:45 min

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October 19, 2016

DOI:

10.3791/54715-v

DOI:
10.3791/54715-v

•

09:45 min
October 19, 2016

•

1 Views
, , ,
1Laboratory of Lymphocyte Biology,Rockefeller University, 2Department of Biology,Harvey Mudd College, 3Masters School

Tags

Trypanosoma BruceiVariant Surface GlycoproteinVSG SwitchingMagnetic Activated Cell SortingFlow CytometryAntigenic VariationCell IsolationCell EnrichmentLister 427 StrainAnti-VSG AntibodyCell SeparationCell CultureCell CentrifugationCell LabelingMagnetic Beads

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Schulz, D., Mugnier, M. R., Boothroyd, C. E., Papavasiliou, F. N. Detection of Trypanosoma brucei Variant Surface Glycoprotein Switching by Magnetic Activated Cell Sorting and Flow Cytometry. J. Vis. Exp. (116), e54715, doi:10.3791/54715 (2016).

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