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Immunology and Infection

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JoVE Journal Immunology and Infection
Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function
 

Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function

Article DOI: 10.3791/54698-v • 11:50 min • November 4th, 2016
November 4th, 2016 •
1,2, 3, 2, 1
1Department of Comparative Biomedical Sciences, The Royal Veterinary College, 2Institute of Physiology I, Cardiology & Vascular Medicine, Eberhard Karls University of Tuebingen, 3Department of Clinical Science and Services, The Royal Veterinary College

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Many experimental systems have been utilized to understand the mechanisms regulating T cell development and function in an immune response. Here a genetic approach using retroviral transduction is described, which is economic, time efficient, and most importantly, highly informative in identifying regulatory pathways.

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Keywords: Retroviral Transduction Helper T Cells Genetic Approach T Cell Differentiation T Cell Function MicroRNA Regulation HEK293T Cells Calcium Phosphate Transfection
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