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인간의 기본 세포 배양의 일주기 시계 유전자 발현 및 호르몬 분비의 병렬 측정
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November 11, 2016
DOI:
Summary
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Here, we describe settings to monitor in parallel circadian bioluminescence and the secretory activity of human islet cells and primary myotubes. For this, we employed lentiviral gene delivery of a luciferase core clock reporter, followed by in vitro synchronization and collection of outflow medium by continuous cell perifusion.
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Petrenko, V., Saini, C., Perrin, L., Dibner, C. Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures. J. Vis. Exp. (117), e54673, doi:10.3791/54673 (2016).
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