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ヒト初代細胞培養物における概日時計遺伝子発現やホルモン分泌のパラレル測定
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November 11, 2016
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Here, we describe settings to monitor in parallel circadian bioluminescence and the secretory activity of human islet cells and primary myotubes. For this, we employed lentiviral gene delivery of a luciferase core clock reporter, followed by in vitro synchronization and collection of outflow medium by continuous cell perifusion.
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Petrenko, V., Saini, C., Perrin, L., Dibner, C. Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures. J. Vis. Exp. (117), e54673, doi:10.3791/54673 (2016).
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