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Ganglio espiral neurona explante cultura y Electrofisiología de multi electrodos matrices
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Spiral Ganglion Neuron Explant Culture and Electrophysiology on Multi Electrode Arrays
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Ganglio espiral neurona explante cultura y Electrofisiología de multi electrodos matrices

9,589 Views

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07:51 min

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October 19, 2016

DOI:

10.3791/54538-v

DOI:
10.3791/54538-v

•

07:51 min
October 19, 2016

•

11 Views
, , , , ,
1Department of Otorhinolaryngology,Inselspital and University of Bern, 2Department of Clinical Research,Inselspital and University of Bern, 3Department of Physiology,University of Bern, 4Department of Clinical Neurosciences, Service ORL & HNS,University Hospital Geneva

Summary

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We present a protocol to culture primary murine spiral ganglion neuron explants on multi electrode arrays to study neuronal response profiles and optimize stimulation parameters. Such studies aim to improve the neuron-electrode interface of cochlear implants to benefit hearing in patients as well as the energy consumption of the device.

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Hahnewald, S., Roccio, M., Tscherter, A., Streit, J., Ambett, R., Senn, P. Spiral Ganglion Neuron Explant Culture and Electrophysiology on Multi Electrode Arrays. J. Vis. Exp. (116), e54538, doi:10.3791/54538 (2016).

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