Here, we present a protocol to more safely and efficiently administer anesthetic gas to mice using a digital, low flow anesthesia system utilizing a syringe-driven direct injection vaporizer.
Adelsperger, A. R., Bigiarelli-Nogas, K. J., Toore, I., Goergen, C. J. Use of a Low-flow Digital Anesthesia System for Mice and Rats. J. Vis. Exp. (115), e54436, doi:10.3791/54436 (2016).