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Genomweite Reinigung von extrachromosomale zirkuläre DNA aus eukaryotischen Zellen
Journal JoVE
Biologie
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Journal JoVE Biologie
Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells
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Genomweite Reinigung von extrachromosomale zirkuläre DNA aus eukaryotischen Zellen

24,578 Views

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14:26 min

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April 04, 2016

DOI:

10.3791/54239-v

DOI:
10.3791/54239-v

•

14:26 min
April 04, 2016

•

45 Views
, , , , ,
1Department of Biology,University of Copenhagen, 2National Veterinary Institute,Technical University of Denmark, 3Group Health Research Institute, 4Lewis-Sigler Institute for Integrative Genomics,Princeton University, 5Calico Life Sciences LLC

Summary

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This paper presents a sensitive method called Circle-Seq for purifying extrachromosomal circular DNA (eccDNA). The method encompasses column purification, removal of remaining linear chromosomal DNA, rolling-circle amplification and high-throughput sequencing. Circle-Seq is applicable to genome-scale screening of eukaryotic eccDNA and studying genome instability and copy-number variation.

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Cite this Article

Møller, H. D., Bojsen, R. K., Tachibana, C., Parsons, L., Botstein, D., Regenberg, B. Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells. J. Vis. Exp. (110), e54239, doi:10.3791/54239 (2016).

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