Journal
/
/
जनरेशन और जीएम-सीएसएफ की पहचान व्युत्पन्न वायुकोशीय तरह मैक्रोफेज और वृक्ष के समान माउस अस्थि मज्जा से कोशिकाओं
Journal JoVE
Immunologie et infection
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
Generation and Identification of GM-CSF Derived Alveolar-like Macrophages and Dendritic Cells From Mouse Bone Marrow
DOI:

11:05 min

June 25, 2016

, , , , ,

Chapitres

  • 00:05Titre
  • 01:11Murine Femur and Tibia Single Bone Marrow Suspesion Preparation
  • 05:55Bone Marrow Cell (BMC) Culture
  • 08:39Results: Representative Bone Marrow Dendritic Cell Culture Analyses
  • 10:13Conclusion

Summary

Traduction automatique

Bone marrow cells cultured with granulocyte macrophage colony stimulating factor (GM-CSF) generate a heterogeneous culture containing macrophages and dendritic cells (DCs). This method highlights using MHCII and hyaluronan (HA) binding to differentiate macrophages from the DCs in the GM-CSF culture. Macrophages in this culture have many similarities to alveolar macrophages.

Vidéos Connexes

Read Article