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La combinazione di doppio Fluorescenza<em> In Situ</em> Ibridazione con Immunolabelling per il rilevamento della Manifestazione di tre geni nel cervello di topo Sezioni
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Chapitres
- 00:05Titre
- 00:42Overview of Double Fluoresence In Situ Hybridization Followed by Immunostaining
- 01:44RNA Probe Synthesis
- 04:12Cryosectioning and Hybridization
- 05:33Visualization of the FITC Probe and the DIG Probe
- 07:23Immunohistochemistry and Mounting
- 08:16Results: Expression of Aspa in Oligodendrocytes in the Cortex and the Corpus Callosum
- 08:45Conclusion
Localizing gene expression to specific cell types can be challenging due to the lack of specific antibodies. Here we describe a protocol for simultaneous triple detection of gene expression by combining double fluorescence RNA in situ hybridization with immunostaining.
Tags
Keywords:
Double Fluorescence In Situ HybridizationImmunolabellingGene ExpressionMouse Brain SectionsCell Type LocalizationRNA ProbesFITCDIGTyramideAlkaline PhosphataseFast RedImmunostainingPlasmid DNARNA Polymerase PromotersCDNARestriction EnzymeLinearizationPurificationPhenol-chloroform ExtractionEthanol Precipitation
