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Heterotopic Renal Autotransplantation in a Porcine Model
 

Heterotopic Renal Autotransplantation in a Porcine Model: A Step-by-Step Protocol

Article DOI: 10.3791/53765-v • 11:47 min • February 21st, 2016
February 21st, 2016 •
1,2, 1,3, 1, 1, 4, 4, 5, 1, 6, 1, 2
1Multi Organ Transplant Program, Department of Surgery, Toronto General Hospital, 2Division of Nephrology, The Hospital for Sick Children, 3Programa de Doctorat en Medicina, La Universitat Autónoma de Barcelona, 4Laboratory Medicine and Pathobiology, Toronto General Hospital, 5Department of Medicine, Toronto General Hospital, 6Departments of Surgery (Urology) & Physiology, Developmental & Stem Cell Biology, The Hospital for Sick Children

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Porcine models of organ transplantation provide an important platform to study mechanisms of organ preservation. This article describes a heterotopic porcine renal autotransplantation model, which allows investigating new approaches to improve the outcome of transplantation using marginal kidney grafts.

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Heterotopic Renal Autotransplantation Porcine Model Surgical Procedure Renal Graft Function Protocol Video Manuscript Key Actions Porcine Model Differences Anesthesia Intubation Laryngoscope Lidocaine Solution Cuff Blockage Vaporizer Regulation Jugular Vein Catheterization Seldinger Technique Guidewire Peel-away Introducer Catheter Securing Antibiotics Analgesics
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