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Dyrkning Menneskelig pluripotente og neurale stamceller i en Lukket Cell Culture System for Basic og Prækliniske Research
Journal JoVE
Biologie du développement
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Journal JoVE Biologie du développement
Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research

Dyrkning Menneskelig pluripotente og neurale stamceller i en Lukket Cell Culture System for Basic og Prækliniske Research

DOI:
10.3791/53685-v

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08:05 min

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June 10, 2016

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, , , , ,
1National Human Neural Stem Cell Resource,Childrens Hospital of Orange County Research Institute

Chapitres

  • 00:05Titre
  • 00:54Setting Gas Concentrations and Temperature
  • 02:34Operation of Buffer Chambers
  • 03:53Incubator Setup and Cell Production Facility Preparation
  • 05:36Feeding Cell Cultures
  • 06:23Results: Pluripotent Stem Cells Derived in Hypoxia with Sendai Virus
  • 07:13Conclusion

Summary

Traduction automatique

Traduction automatique

Here is a protocol to grow pluripotent stem cells (PSC) and neural stem cells (NSC) in an enclosed cell culture system that permits maximum sterility and reproducibility, replacing the traditional biosafety cabinet and incubator. This equipment meets clinical good manufacturing practice (cGMP) and clinical good lab practice (cGLP) guidelines.

Tags

CulturingHuman Pluripotent Stem CellsNeural Stem CellsEnclosed Cell Culture SystemBasic ResearchPreclinical ResearchStem Cell GrowthDefined Atmospheric ConditionsSterilityCell Culture ParametersMultipotent Stem CellsPluripotent Stem CellsConstant EnvironmentOxygen ConcentrationCarbon Dioxide ConcentrationGas Concentrations

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