Here, we present an overview of the preparation and animal handling procedures required to safely perform medical imaging in an animal biosafety level 4 laboratory. Computed tomography of a mock-infected guinea pig illustrates these procedures that may be used to evaluate the disease caused by a high consequence pathogen.
Byrum, R., Keith, L., Bartos, C., St. Claire, M., Lackemeyer, M. G., Holbrook, M. R., Janosko, K., Barr, J., Pusl, D., Bollinger, L., Wada, J., Coe, L., Hensley, L. E., Jahrling, P. B., Kuhn, J. H., Lentz, M. R. Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 4. Medical Imaging Procedures. J. Vis. Exp. (116), e53601, doi:10.3791/53601 (2016).