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網膜リボンシナプスにおける膜受容体の高分解能定量的イムノゴールド分析
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Neurosciences
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Journal JoVE Neurosciences
High-Resolution Quantitative Immunogold Analysis of Membrane Receptors at Retinal Ribbon Synapses
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網膜リボンシナプスにおける膜受容体の高分解能定量的イムノゴールド分析

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09:24 min

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February 18, 2016

DOI:

10.3791/53547-v

DOI:
10.3791/53547-v

•

09:24 min
February 18, 2016

•

2 Views
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1Synaptic Physiology Section, National Institute of Neurological Disorders and Stroke,National Institutes of Health, 2Advanced Imaging Core, National Institute on Deafness and Other Communication Disorders,National Institutes of Health

Summary

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The postembedding immunogold method is one of the most effective ways to provide high-resolution analyses of the subcellular localization of specific molecules. Here we describe a protocol to quantitatively analyze glutamate receptors at retinal ribbon synapses.

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Quantitative Immunogold AnalysisMembrane ReceptorsRetinal Ribbon SynapsesFreeze-substitutionUltra-thin SectioningRetinal NeurobiologyProtein LocalizationProtein DensityRetinal Circuit

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Zhang, J., Petralia, R. S., Wang, Y., Diamond, J. S. High-Resolution Quantitative Immunogold Analysis of Membrane Receptors at Retinal Ribbon Synapses. J. Vis. Exp. (108), e53547, doi:10.3791/53547 (2016).

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