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Ein Semi-automatisierten Ansatz zur Vorbereitung Antikörper-Cocktails für immunphänotypische Analyse von menschlichem peripherem Blut
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood
DOI:

08:17 min

February 08, 2016

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Chapitres

  • 00:05Titre
  • 00:58Operating Procedure
  • 06:25Results: Low Variability in Semi-automated Whole Blood Immunophenotyping
  • 07:04Conclusion

Summary

Traduction automatique

Whole blood Immunophenotyping is indispensable for monitoring the human immune system. However, the number of reagents required and the instability of specific fluorochromes in premixed cocktails necessitates daily reagent preparation. Here we show that semi-automated preparation of staining antibody cocktail helps establish reliable immunophenotyping by minimizing variability in reagent dispensing.

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