半自动化的方法准备抗体鸡尾酒人外周血中免疫表型分析
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February 08, 2016
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Whole blood Immunophenotyping is indispensable for monitoring the human immune system. However, the number of reagents required and the instability of specific fluorochromes in premixed cocktails necessitates daily reagent preparation. Here we show that semi-automated preparation of staining antibody cocktail helps establish reliable immunophenotyping by minimizing variability in reagent dispensing.
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Koguchi, Y., Gonzalez, I. L., Meeuwsen, T. L., Miller, W. L., Haley, D. P., Tanibata-Branham, A. N., Bahjat, K. S. A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood. J. Vis. Exp. (108), e53485, doi:10.3791/53485 (2016).
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