Journal
/
/
세포 외 산 생산의 측정 및 분석은 당분 속도를 확인하는
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate

세포 외 산 생산의 측정 및 분석은 당분 속도를 확인하는

24,630 Views

06:47 min

December 12, 2015

DOI:

06:47 min
December 12, 2015

5 Views
,

Summary

Automatically generated

Glycolysis is a defining metabolic marker in multiple biological systems. Monitoring glycolysis by measuring the extracellular flux of H+ is common, but requires correction to be quantitative and unambiguous. Here, we demonstrate how to gather and correct extracellular flux data to distinguish between respiratory and glycolytic sources of extracellular acidification.

Read Article