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Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
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Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

DOI:
10.3791/53462-v

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06:25 min

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April 26, 2016

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, , , , , ,
1School of Science and Health,University of Western Sydney, 2Van′t Hoff Institute for Molecular Sciences,University of Amsterdam

Chapitres

  • 00:05Titre
  • 01:13Detection of ROS Using DPPH
  • 04:08Results: Post Column Derivatization Using High Performance Liquid Chromatography
  • 05:41Conclusion

Summary

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A protocol for the use of reaction flow high performance liquid chromatography columns for methods employing post column derivatization (PCD) is presented.

Tags

Post Column DerivatizationReaction FlowHigh Performance Liquid ChromatographyHPLCPCDUV/VIS DetectorDPPHAntioxidantsAmino AcidsPhenolsThiolsMetalsAntibioticsToxinsColumn EquilibrationFlow RateMethanolSensitivityEfficiency

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