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Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models
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Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models
Chapitres
- 00:05Titre
- 00:33Intestine Dissection
- 01:32Formalin Fixation
- 02:25Methacarn Fixation
- 03:38Whole Mount LacZ Visualization
- 06:01Results: Representative Intestinal Epithelial Cell Characterization After Gene Manipulation
- 07:20Conclusion
Intestinal epithelial stem cells (ISCs) are intermingled with Paneth cells. These cells are differentiated progeny of the ISC, which support the ISCs and provide antibacterial protection. Here we demonstrate how we used transgenic conditional mouse models to establish that Paneth cells play a crucial role in maintaining the intestinal epithelia.
