Journal
/
Biologie
/
Generazione e Cultura Sangue escrescenza endoteliali cellule da sangue periferico umano
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Generation and Culture of Blood Outgrowth Endothelial Cells from Human Peripheral Blood
Please note that all translations are automatically generated. Click here for the English version.

Generazione e Cultura Sangue escrescenza endoteliali cellule da sangue periferico umano

18,553 Views

•

11:00 min

•

December 23, 2015

DOI:

10.3791/53384-v

DOI:
10.3791/53384-v

•

11:00 min
December 23, 2015

•

9 Views
, , , , , ,
1Department of Medicine,University of Cambridge, 2Papworth Hospital

Summary

Automatically generated

This protocol allows for the reliable generation and characterization of blood outgrowth endothelial cells (BOECs) from a small volume of adult peripheral blood. BOECs can be used as a surrogate for endothelial cells from patients with vascular disorders and as a substrate for the generation of induced pluripotent stem cells.

Explore More Videos

Blood Outgrowth Endothelial CellsBOECsEndothelial ProgenitorsEndothelial DysfunctionVascular DisordersPrimary Endothelial CellsCell CulturePatient-derivedEndothelial Cell TransplantationInduced Pluripotent Stem CellsIPSCsNuclear ReprogrammingPeripheral Blood

Read Article

Cite this Article

Ormiston, M. L., Toshner, M. R., Kiskin, F. N., Huang, C. J. Z., Groves, E., Morrell, N. W., Rana, A. A. Generation and Culture of Blood Outgrowth Endothelial Cells from Human Peripheral Blood. J. Vis. Exp. (106), e53384, doi:10.3791/53384 (2015).

Download .ris file

Copy

Share Video

.

Copy