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Immunologie et infection
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감염증에 대한 모델로 Organoids : 헬리코박터 파이로리의 인간의 문화와 쥐 위장 Organoids 및 미세 주입
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Immunologie et infection
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Journal JoVE Immunologie et infection
Organoids as Model for Infectious Diseases: Culture of Human and Murine Stomach Organoids and Microinjection of Helicobacter Pylori
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감염증에 대한 모델로 Organoids : 헬리코박터 파이로리의 인간의 문화와 쥐 위장 Organoids 및 미세 주입

34,471 Views

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10:30 min

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November 12, 2015

DOI:

10.3791/53359-v

DOI:
10.3791/53359-v

•

10:30 min
November 12, 2015

•

36 Views
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1Hubrecht Institute for Developmental Biology and Stem Cell Research,University Medical Centre Utrecht

Summary

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Stem cell derived cultures harbor tremendous potential to model infectious diseases. Here, the culture of mouse and human gastric organoids derived from adult stem cells is described. The organoids are microinjected with the gastric pathogen Helicobacter pylori.

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OrganoidsGastric OrganoidsHelicobacter PyloriHost-pathogen InteractionsStem CellsInfectious DiseasesCultureMicroinjectionImaging

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Bartfeld, S., Clevers, H. Organoids as Model for Infectious Diseases: Culture of Human and Murine Stomach Organoids and Microinjection of Helicobacter Pylori. J. Vis. Exp. (105), e53359, doi:10.3791/53359 (2015).

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