Journal
/
/
피더가없는 조건에 센다이 바이러스를 사용하여 인간 말초 T 세포로부터 유도 된 다 능성 줄기 세포의 생성
Journal JoVE
Biologie du développement
This content is Free Access.
Journal JoVE Biologie du développement
Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions
DOI:

09:32 min

November 11, 2015

, , , ,

Chapitres

  • 00:05Titre
  • 01:19Preparation of Activated Human T Cells
  • 04:05Generating Induced Pluripotent Stem Cells (iPSCs) from Activated T Cells
  • 07:11Expansion of T Cell-derived iPSCs
  • 08:28Results: Generation of iPSCs from Human Peripheral T Cells
  • 09:03Conclusion

Summary

Traduction automatique

This protocol describes how to generate induced pluripotent stem cells (iPSCs) from human peripheral T cells in feeder-free conditions using a combination of matrigel and Sendai virus vectors containing reprogramming factors.

Vidéos Connexes

Read Article