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العدوى فيفو السابقين الفئران البشرة مع فيروس الهربس البسيط من النوع 1
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Immunologie et infection
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Journal JoVE Immunologie et infection
Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1
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العدوى فيفو السابقين الفئران البشرة مع فيروس الهربس البسيط من النوع 1

10,964 Views

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11:56 min

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August 24, 2015

DOI:

10.3791/53046-v

DOI:
10.3791/53046-v

•

11:56 min
August 24, 2015

•

22 Views
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1Center for Biochemistry,University of Cologne, 2Department of Dermatology,University of Cologne

Summary

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The skin is one target tissue of the human pathogen herpes simplex virus type 1 (HSV-1). To explore the invasion route of HSV-1 into tissue, we established an ex vivo infection model of murine epidermal sheets which represent the outermost layer of skin.

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Ex VivoMurine EpidermisHerpes Simplex Virus Type 1HSV-1KeratinocytesPrimary InfectionLatent InfectionReactivationICP0Nectin-1HVEMCell-to-cell SpreadVirus Production

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Cite this Article

Rahn, E., Thier, K., Petermann, P., Knebel-Mörsdorf, D. Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1. J. Vis. Exp. (102), e53046, doi:10.3791/53046 (2015).

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