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Immunologie et infection
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Cuantificación de Citosólica vs. Vacuolar<em> Salmonella</em> En macrófagos primarios por permeabilización diferencial
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Immunologie et infection
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Journal JoVE Immunologie et infection
Quantification of Cytosolic vs. Vacuolar Salmonella in Primary Macrophages by Differential Permeabilization
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Cuantificación de Citosólica vs. Vacuolar<em> Salmonella</em> En macrófagos primarios por permeabilización diferencial

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09:25 min

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July 28, 2015

DOI:

10.3791/52960-v

DOI:
10.3791/52960-v

•

09:25 min
July 28, 2015

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4 Views
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1Focal Area Infection Biology, Biozentrum,University of Basel

Summary

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We describe the quantification of cytosolic and vacuolar Salmonella typhimurium in bone-marrow derived macrophages using differential digitonin permeabilization.

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SalmonellaIntracellular BacteriaCytosolVacuoleSalmonella-containing Vacuole (SCV)Differential PermeabilizationPhagosome-protection AssayDigitoninFluorescence MicroscopyFlow Cytometry

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Cite this Article

Meunier, E., Broz, P. Quantification of Cytosolic vs. Vacuolar Salmonella in Primary Macrophages by Differential Permeabilization. J. Vis. Exp. (101), e52960, doi:10.3791/52960 (2015).

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