Neutrophils play an important role not only in host defense against invading microorganisms, but are also involved in the immune surveillance of tumor cells. Here, we describe techniques related to the isolation of neutrophils with anti-tumor properties and methods for monitoring anti-tumor neutrophil function in vitro and in vivo.
Sionov, R. V., Assi, S., Gershkovitz, M., Sagiv, J. Y., Polyansky, L., Mishalian, I., Fridlender, Z. G., Granot, Z. Isolation and Characterization of Neutrophils with Anti-Tumor Properties. J. Vis. Exp. (100), e52933, doi:10.3791/52933 (2015).