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Developmental Biology

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JoVE Journal Developmental Biology
Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids
 

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids

Article DOI: 10.3791/52885-v • 10:52 min • June 5th, 2015
June 5th, 2015 •
*1, *1, 1, 1, 2, 2, 3, 1, 1, 1
1Center for Biomedicine, European Academy Bozen/Bolzano (EURAC), 2Laboratory of Medical Genetics, Fondazione IRCCS Ca´ Granda, Ospedale Maggiore Policlinico, 3Del E. Webb Center for Neuroscience, Aging & Stem Cell Research, Sanford-Burnham Medical Research Institute
* These authors contributed equally

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résumé
Automatic Translation

Induced pluripotent stem cells (iPSCs) represent a source of patient-specific tissues for clinical applications and basic research. Here, we present a detailed protocol to reprogram human peripheral blood mononuclear cells (PBMNCs) obtained from frozen buffy coats into viral-free iPSCs using non-integrating episomal plasmids.

Tags

Induced Pluripotent Stem Cells IPSCs Transcription Factors Oct-4 Sox-2 Klf-4 C-Myc Human Embryonic Stem Cells HESCs Regenerative Medicine Ethical Issues Transgene-free IPSCs Gene Expression Differentiation Non-integrating Episomal Plasmids Reprogramming Method Fibroblasts Tissue Biopsy Human Peripheral Blood Mononuclear Cells PBMNCs
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