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Påvisning af sygdom-associeret α-synuclein ved Enhanced ELISA i hjernen hos transgene mus overudtrykker Menneskelig A53T Muterede α-synuclein
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Médecine
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Journal JoVE Médecine
Detection of Disease-associated α-synuclein by Enhanced ELISA in the Brain of Transgenic Mice Overexpressing Human A53T Mutated α-synuclein

Påvisning af sygdom-associeret α-synuclein ved Enhanced ELISA i hjernen hos transgene mus overudtrykker Menneskelig A53T Muterede α-synuclein

DOI:
10.3791/52752-v

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12:01 min

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May 30, 2015

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, , , , , , , ,
1Neurodegenerative Diseases Unit,ANSES – French Agency for Food, Environmental and Occupational Health & Safety, 2Indicia Production, 3AJ Roboscreen GmbH, 4Epidemiology Unit,ANSES – French Agency for Food, Environmental and Occupational Health & Safety, 5Experimental Facilities,ANSES – French Agency for Food, Environmental and Occupational Health & Safety

Chapitres

  • 00:05Titre
  • 02:32Dissect Brain Regions
  • 04:53Prepare Brain Homogenates
  • 06:02Detection of αSD by ELISA
  • 08:49Results: Disease Detection Using Enhanced ELISA
  • 10:39Conclusion

Summary

Traduction automatique

Traduction automatique

An ELISA offering a novel quantitative approach is described. It specifically detects disease-associated α-synuclein (αSD) in a transgenic mouse model (M83) of synucleinopathy using several antibodies against either the Ser129 phosphorylated αS form or the C-terminal part of the protein.

Tags

Keywords: α-synucleinDisease-associated α-synucleinELISATransgenic MouseA53T MutationSynucleopathiesQuantificationWestern BlotImmunohistochemistrySer129 PhosphorylationPrion-like Mechanism

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