We present a protocol for capturing the dynamics of zebrafish larval tail fin regeneration on a whole-tissue scale using brightfield-based stereomicroscopy. This technique enables capturing the regeneration dynamics with single cell resolution. This methodology can be adapted to any stereomicroscope equipped with a CCD camera and time-lapse software.
Lisse, T. S., Brochu, E. A., Rieger, S. Capturing Tissue Repair in Zebrafish Larvae with Time-lapse Brightfield Stereomicroscopy. J. Vis. Exp. (95), e52654, doi:10.3791/52654 (2015).