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JoVE Journal
Immunology and Infection

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Loop-mediated Isothermal Amplification (LAMP) Assays for the Species-specific Detection of Eimeria that Infect Chickens
 

Loop-mediated Isothermal Amplification (LAMP) Assays for the Species-specific Detection of Eimeria that Infect Chickens

Article DOI: 10.3791/52552-v 06:57 min February 20th, 2015
February 20th, 2015

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résumé

Diagnosis of Eimeria infection in chickens remains demanding. Parasite morphology- and host pathology-led approaches are commonly inconclusive, while molecular approaches based on PCR have proven demanding in cost and expertise. The aim of this protocol is to establish loop-mediated isothermal amplification (LAMP) as a straightforward molecular diagnostic for eimerian infection.

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Eimeria Coccidiosis LAMP Assay Species-specific Detection Chicken Intestinal Tissue Genomic DNA Molecular Diagnosis
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