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转染，选择和人类诱导多能干细胞集落采摘TALEN靶向用GFP基因进入AAVS1安全港
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Biologie du développement
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Journal JoVE Biologie du développement
Transfection, Selection, and Colony-picking of Human Induced Pluripotent Stem Cells TALEN-targeted with a GFP Gene into the AAVS1 Safe Harbor
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转染，选择和人类诱导多能干细胞集落采摘TALEN靶向用GFP基因进入AAVS1安全港

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07:28 min

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February 01, 2015

DOI:

10.3791/52504-v

DOI:
10.3791/52504-v

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07:28 min
February 01, 2015

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10 Views
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1National Institute of Arthritis, Musculoskeletal and Skin Diseases,National Institutes of Health, 2Q Therapeutics

Tags

TransfectionSelectionColony-pickingHuman Induced Pluripotent Stem CellsTALENGFPAAVS1 Safe HarborTargeted Transgene AdditionGene EditingGene SilencingInsertional MutagenesisViral VectorGene-trap-like DonorSingle Cell DissociationNuclear Delivery EfficiencyPuromycin SelectionPPP1R12CSplicing AcceptorPACTargeted ClonesGenetic ScreeningFunctional ScreeningGFP Reporter IPSC Lines

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Cerbini, T., Luo, Y., Rao, M. S., Zou, J. Transfection, Selection, and Colony-picking of Human Induced Pluripotent Stem Cells TALEN-targeted with a GFP Gene into the AAVS1 Safe Harbor. J. Vis. Exp. (96), e52504, doi:10.3791/52504 (2015).

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