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Investigating Single Molecule Adhesion by Atomic Force Spectroscopy
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February 27, 2015
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A protocol to couple a large variety of single molecules covalently onto an AFM tip is presented. Procedures and examples to determine the adhesion force and free energy of these molecules on solid supports and bio-interfaces are provided.
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Stetter, F. W. S., Kienle, S., Krysiak, S., Hugel, T. Investigating Single Molecule Adhesion by Atomic Force Spectroscopy. J. Vis. Exp. (96), e52456, doi:10.3791/52456 (2015).
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