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Human células estaminais pluripotentes Baseado Developmental Toxicity Ensaios para Química Triagem e Safety Systems Biology Data Generation
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Journal JoVE Biologie du développement
Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

Human células estaminais pluripotentes Baseado Developmental Toxicity Ensaios para Química Triagem e Safety Systems Biology Data Generation

12,368 Views

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17:28 min

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June 17, 2015

DOI:

10.3791/52333-v

DOI:
10.3791/52333-v

•

17:28 min
June 17, 2015

•

3 Views
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1Center of Physiology and Pathophysiology, Institute of Neurophysiology,University of Cologne, 2Department of Biology,University of Konstanz, 3Department of Statistics,Technical University of Dortmund, 4Leibniz Research Centre for Working Environment and Human Factors,Technical University of Dortmund

Summary

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Os protocolos descrevem dois sistemas in vitro de testes de toxicidade de desenvolvimento (UKK e UKN1) com base em células estaminais embrionárias humanas e estudos de transcriptoma. Os sistemas de teste prever risco de toxicidade para o desenvolvimento humano, e pode contribuir para reduzir os estudos em animais, dos custos e do tempo necessário para os testes de segurança química.

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Shinde, V., Klima, S., Sureshkumar, P. S., Meganathan, K., Jagtap, S., Rempel, E., Rahnenführer, J., Hengstler, J. G., Waldmann, T., Hescheler, J., Leist, M., Sachinidis, A. Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation. J. Vis. Exp. (100), e52333, doi:10.3791/52333 (2015).

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