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Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles
 

Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles

Article DOI: 10.3791/52323-v • 12:00 min • January 22nd, 2015
January 22nd, 2015 •
1, 1, 2, 2, 1, 1
1Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, 2Laboratory for Biomaterials and Drug Delivery, Department of Anesthesiology, Division of Critical Care, Boston Children's Hospital

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The objective of this protocol is to incorporate SDF-1α, a stem cell homing factor, into dextran sulfate-chitosan nanoparticles. The resultant particles are measured for their size and zeta potential, as well as the content, activity, and in vitro release rate of SDF-1α from the nanoparticles.

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SDF-1α Chitosan-Dextran Sulfate Nanoparticles Polyelectrolyte Complex-based Nanoparticles Protein Delivery In Vivo Delivery Targeted Tissue Aqueous Solution Heparin-binding Site Inflammatory Toxic Stem Cell Homing Factor Polyelectrolyte Complexes Zinc Sulfate Zinc Bridges Size Measurement Surface Charge Measurement SDF-1α Incorporation In Vitro Release Rate Chemotactic Activity
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