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Método de aislados<em> Ex Vivo</em> Perfusión pulmonar en un modelo de rata: Lecciones Aprendidas de Desarrollo de un Programa EVLP Rata
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February 25, 2015
DOI:
1Department of Biomedical Engineering,Ohio State University Wexner Medical Center, 2Davis Heart & Lung Research Institute,Ohio State University Wexner Medical Center, 3The Collaboration for Organ Perfusion, Protection, Engineering and Regeneration (COPPER) Laboratory,Ohio State University Wexner Medical Center, 4Division of Cardiac Surgery, Department of Surgery,Ohio State University Wexner Medical Center, 5Departments of Pediatrics and Internal Medicine,Ohio State University, 6Advanced Lung Disease Program, Lung and Heart-Lung Transplant Programs,Nationwide Children’s Hospital, 7Division of Transplantation, Department of Surgery,Ohio State University Wexner Medical Center
Summary
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Ex-Vivo perfusión pulmonar (EVLP) ha permitido el trasplante de pulmón en los seres humanos para ser más fácilmente disponible, permitiendo la capacidad de evaluar los órganos y ampliar el grupo de donantes. Aquí se describe el desarrollo de un programa EVLP rata y mejoras que permitan un modelo reproducible para una futura expansión.
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Nelson, K., Bobba, C., Eren, E., Spata, T., Tadres, M., Hayes, Jr., D., Black, S. M., Ghadiali, S., Whitson, B. A. Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program. J. Vis. Exp. (96), e52309, doi:10.3791/52309 (2015).
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