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Generación de plásmidos vectores que expresan proteínas de la bandera de etiquetado bajo la regulación del promotor factor de elongación humano-1α Usando Asamblea Gibson
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February 09, 2015
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Summary
Automatically generated
Synthesis of custom plasmids is labor and time consuming. This protocol describes the use of Gibson assembly cloning to reduce the work and duration of custom DNA cloning procedure. The protocol described also produces reliable tagged protein constructs for mammalian expression at similar cost to the traditional cut-and-paste DNA cloning.
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Grozdanov, P. N., MacDonald, C. C. Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly. J. Vis. Exp. (96), e52235, doi:10.3791/52235 (2015).
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