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Herstellung und Verwendung von Lentivirus, um selektiv zu transduzieren Primäre Oligodendrozyten Vorläuferzellen für<em> In-vitro-</em> Myelinisierung Assays
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Biologie du développement
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Journal JoVE Biologie du développement
Production and Use of Lentivirus to Selectively Transduce Primary Oligodendrocyte Precursor Cells for In Vitro Myelination Assays
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Herstellung und Verwendung von Lentivirus, um selektiv zu transduzieren Primäre Oligodendrozyten Vorläuferzellen für<em> In-vitro-</em> Myelinisierung Assays

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10:00 min

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January 12, 2015

DOI:

10.3791/52179-v

DOI:
10.3791/52179-v

•

10:00 min
January 12, 2015

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9 Views
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1Department of Anatomy and Neuroscience,The University of Melbourne, 2The Florey Institute of Neuroscience and Mental Health Research,The University of Melbourne

Summary

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Here we present protocols that offer a flexible and strategic foundation for virally manipulating oligodendrocyte precursor cells to overexpress proteins of interest in order to specifically interrogate their role in oligodendrocytes via the in vitro model of central nervous system myelination.

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LentivirusPrimary Oligodendrocyte Precursor CellsIn Vitro Myelination AssayDorsal Root Ganglion NeuronsOligodendroglial ProteinsMyelinationCentral Nervous SystemProtein OverexpressionSelective Transduction

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Cite this Article

Peckham, H. M., Ferner, A. H., Giuffrida, L., Murray, S. S., Xiao, J. Production and Use of Lentivirus to Selectively Transduce Primary Oligodendrocyte Precursor Cells for In Vitro Myelination Assays. J. Vis. Exp. (95), e52179, doi:10.3791/52179 (2015).

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