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The Analysis of Neurovascular Remodeling in Entorhino-hippocampal Organotypic Slice Cultures
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
The Analysis of Neurovascular Remodeling in Entorhino-hippocampal Organotypic Slice Cultures
DOI:

08:27 min

October 23, 2014

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Chapitres

  • 00:05Titre
  • 01:20Organotypic Entorhino-hippocampal Slice Cultures
  • 02:37In Vitro Hypoxia and Oxygen-glucose Deprivation (OGD) with Reperfusion
  • 04:28Fixation and Immunostaining
  • 05:47Determination of Regional Vessel Density
  • 06:22Results: Neuronal Death and Blood Vessel Loss after Hypoxia or OGD
  • 07:41Conclusion

Summary

Traduction automatique

A protocol for entorhino-hippocampal organotypic slice cultures, which allows reproducing many aspects of ischemic brain injury, is presented. By studying changes of the neurovasculature in addition to changes in the neurons, this protocol is a versatile tool to study plastic changes in neural tissue after injury.

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