Journal
/
/
Gepaart ganze Zelle Recordings in Organotypische Hippocampus
Journal JoVE
Neurosciences
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Neurosciences
Paired Whole Cell Recordings in Organotypic Hippocampal Slices

Gepaart ganze Zelle Recordings in Organotypische Hippocampus

14,449 Views

09:23 min

September 28, 2014

DOI:

09:23 min
September 28, 2014

6 Views
, , ,

Summary

Automatically generated

Pair recordings are simultaneous whole cell patch clamp recordings from two synaptically connected neurons, enabling precise electrophysiological and pharmacological characterization of the synapses between individual neurons. Here we describe the detailed methodology and requirements for establishing this technique in organotypic hippocampal slice cultures in any laboratory equipped for electrophysiology.

Read Article