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Секвенирование РНК анализ дифференциальных экспрессии генов в электропорации Чик эмбрионального спинного мозга
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Neurosciences
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Journal JoVE Neurosciences
RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord
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Секвенирование РНК анализ дифференциальных экспрессии генов в электропорации Чик эмбрионального спинного мозга

14,379 Views

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11:13 min

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November 01, 2014

DOI:

10.3791/51951-v

DOI:
10.3791/51951-v

•

11:13 min
November 01, 2014

•

2 Views
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1Department of Cell and Developmental Biology,Universidade de São Paulo

Summary

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This video shows the basic steps for performing whole transcriptome analysis on dissected chick embryonic spinal cord samples after transfection with in ovo electroporation.

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RNA-SeqDifferential Gene ExpressionChick Embryonic Spinal CordIn Ovo ElectroporationNeural DevelopmentTranscriptome AnalysisGene FunctionBioinformaticsGalaxy Public ServerHigh-quality RNA SamplesOverexpression

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Cite this Article

Vieceli, F. M., Yan, C. I. RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord. J. Vis. Exp. (93), e51951, doi:10.3791/51951 (2014).

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