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Expressão de proteínas recombinantes de Biologia Estrutural em HEK 293F Suspensão Cells: Uma abordagem inovadora e acessível
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach

Expressão de proteínas recombinantes de Biologia Estrutural em HEK 293F Suspensão Cells: Uma abordagem inovadora e acessível

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11:20 min

October 16, 2014

DOI:

11:20 min
October 16, 2014

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Summary

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The expression of recombinant proteins by mammalian systems is becoming an attractive method for producing protein complexes for structural biology. Here we present a simple yet highly efficient expression system using suspension grown mammalian cells to purify protein complexes for structural studies.

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