This content is Free Access.
Expresión de proteínas recombinantes para la Biología Estructural en HEK 293F Suspensión Células: Un enfoque novedoso y accesible
52,615 Views
•
11:20 min
•
October 16, 2014
DOI:
Summary
Automatically generated
The expression of recombinant proteins by mammalian systems is becoming an attractive method for producing protein complexes for structural biology. Here we present a simple yet highly efficient expression system using suspension grown mammalian cells to purify protein complexes for structural studies.
Read Article
Cite this Article
Portolano, N., Watson, P. J., Fairall, L., Millard, C. J., Milano, C. P., Song, Y., Cowley, S. M., Schwabe, J. W. Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach. J. Vis. Exp. (92), e51897, doi:10.3791/51897 (2014).
Copy