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دليل ل<em> في الجسم الحي</em> تسجيل وحدة واحدة من التي تم تحديدها Optogenetically القشرية التثبيطي Interneurons
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Neurosciences
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Journal JoVE Neurosciences
A Guide to In vivo Single-unit Recording from Optogenetically Identified Cortical Inhibitory Interneurons

دليل ل<em> في الجسم الحي</em> تسجيل وحدة واحدة من التي تم تحديدها Optogenetically القشرية التثبيطي Interneurons

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10:32 min

November 07, 2014

DOI:

10:32 min
November 07, 2014

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Summary

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Here we describe our strategy for obtaining stable, well-isolated single-unit recordings from identified inhibitory interneurons in the anesthetized mouse cortex. Neurons expressing ChR2 are identified by their response to blue light. The method uses standard extracellular recording equipment, and serves as an inexpensive alternative to calcium imaging or visually-guided patching.

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